Skip to main content
. 2018 Aug 8;10(8):414. doi: 10.3390/v10080414

Figure 3.

Figure 3

Microarray analysis of HCV antigen-regulated host genes in human moDCs infected with MVA-HCV and MVA-HCV ∆C6L. (A) Clustering classification of host genes differentially regulated in MVA-WT-, MVA-HCV-, and MVA-HCV ΔC6L-infected human moDCs versus mock infected cells (FDR Prod p value < 0.05, fold change ≥2 or ≤−2). MoDCs were infected for 6 h and the expression levels of different host genes were analyzed using a human oligo microarray 4 × 44 K, as described in Material and Methods. Differentially expressed genes were evaluated by the non-parametric algorithm “Rank Products” available as “RankProd” package at Bioconductor. Results are the mean obtained from three different healthy blood donors. (B) Validation of microarray data by real-time RT-PCR to determine the mRNA levels of IFN-β, IFIT1, and IFIT2 genes. RNA isolated from the three healthy blood donors used for microarray analysis was used. A.U., arbitrary units. Data are means ± SD of duplicate samples. p values indicate significant response differences comparing MVA-HCV and MVA-HCV ΔC6L with MVA-WT (* p < 0.05, ** p < 0.005, *** p < 0.001).