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. 2018 Aug 8;10(8):414. doi: 10.3390/v10080414

Figure 6.

Figure 6

Magnitude, breath, polyfunctionality, and phenotype of HCV-specific CD8+ T cell memory immune responses. Splenocytes were obtained from mice (n = 4 per group) immunized with two doses of MVA-WT, MVA-HCV, or MVA-HCV ∆C6L 53 days after the last immunization. Then, HCV-specific CD8+ T cell memory immune responses elicited were measured by ICS after stimulation of splenocytes with different HCV peptide pools. Values from unstimulated controls were subtracted in all cases. p values indicate significantly response differences when comparing MVA-HCV with MVA-HCV ΔC6L (*** = p ≤ 0.001). Data is from one experiment representative of four independent experiments. (A) Percentage of total HCV-specific CD8+ T cell memory immune responses directed against HCV antigens. The values represent the sum of the percentages of CD8+ T cells producing CD107a and/or IFN-γ and/or TNF-α and/or IL-2 against Core, E1, E2, p7 + NS2, NS3, NS4, and NS5 peptide pools. (B) Percentages of Core, E1, E2, p7 + NS2, NS3, NS4, or NS5 HCV-specific CD8+ T cells. Frequencies represent the sum of the percentages of CD8+ T cells producing CD107a and/or IFN-γ and/or TNF-α and/or IL-2 against each HCV peptide pool. (C) Polyfunctionality profile of total HCV-specific CD8+ T cell memory immune responses directed against HCV antigens and producing CD107a and/or IFN-γ and/or TNF-α and/or IL-2. Responses are grouped and color coded, taking into consideration the number of functions (one, two, three, or four). Each slice in the pie charts corresponds to the proportion of the total HCV-specific CD8+ T cells exhibiting one, two, three, or four functions (CD107a and/or IFN-γ and/or TNF-α and/or IL-2) within the total HCV-specific CD8+ T cells. (D) Phenotypic profile of total HCV-specific CD8+ T cell memory immune responses directed against HCV antigens. Frequencies represent percentages of T central memory (TCM), T effector memory (TEM), and T effector (TE) HCV-specific CD8+ T cells producing CD107a and/or IFN-γ and/or TNF-α and/or IL-2 against HCV peptide pools. TCM, TEM, and TE phenotypes are determined on the basis of CD127 and CD62L expression: TCM (CD127+, CD62L+), TEM (CD127+, CD62L−), and TE (CD127−, CD62L−).