Figure 7.

Quantitative results of the distribution and association of TSP1 in the striatum and cortex. (A). Examples (in the striatum) of correctly identified neurons and astrocytes that associate with TSP1. Scale bars are 200 µm. (B) Percentage of the total identified cell population that were classified as neurons (42.54%, 43.67%) and astrocytes (17.37%, 10.08%) in the striatum and the cortex, respectively. (C) Percent area covered by each phenotypic stain, NeuN (3.88%, 7.04%) and GFAP (4.69%, 3.83%) in the striatum and the cortex, respectively. The percent area covered by NeuN is significantly different in the striatum versus the cortex (p<0.0332). (D) Anterior to posterior distribution of the percent area covered by the TSP1 stain in the striatum and the cortex. Distributions were not significantly different both anterior to posterior and between regions. (E) Average of the percent area covered by TSP1 (8.42%, 7.40%) in the striatum and cortex, respectively. (F) Anterior to posterior distribution of the percentage of phenotypes that associated with TSP1 in both the striatum and the cortex. Distributions were not significantly different anterior to posterior. (G) Averages of the percentage of phenotypes, neurons (94.58%, 88.45%) and astrocytes (47.55%, 28.09%) that associated with TSP1 in the striatum and cortex, respectively. The percentage of astrocytes that associate with TSP1 is significantly different in the striatum versus the cortex (p<0.0332). All error bars represent the standard deviation. Abbreviations: GFAP, glial fibrially acid protein; TSP1, thrombospondin 1.