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. 2018 Jul 31;10(8):404. doi: 10.3390/v10080404

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gene synteny comparisons between previously described Microviridae genomes (left) and those discovered in the Ciona gut (right; CGM). All genomes were manually annotated to start at the major capsid protein (MCP) and open reading frames (ORFs) >80 aa are shown in linear fashion (i.e., overlapping genes are shown in order based on the position of the start codon). ORFs are color-coded based on PHA numbers (the phage protein subset of the Entrez protein cluster (PRK) database). One representative of each gene order known to exist within a given (proposed) subfamily is shown, and the numbers of CGM genomes containing a particular gene order are specified on the far right. The novel CGM group does not have representatives in the database, while the Aravirinae, Stokavirinae, Sukshmavirinae, and Bullavirinae were not detected among the CGM sequences. Details on the gene order for each CGM genome are available in Supplementary Table S2.