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. 2018 Aug 6;9(8):399. doi: 10.3390/genes9080399

Figure 1.

Figure 1

Yeast two-hybrid results showing OFP1–ATH1 interactions. (A) Diagram of constructs used in yeast two-hybrid assays. (B) ATH1 interacts with OFP1 in yeast cells. The protein interaction was detected on synthetic defined quadruple dropout (SD QDO) medium (left) by measuring yeast cell growth state after the yeast cells had been screened and had positive growth on SD double dropout (SD DDO) medium (right) (QDO lacks His, Leu, Ade, and Trp; DDO lacks Trp and Leu). BD, DNA-binding domain; AD, activation domain.