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. 2018 Aug 6;9(8):399. doi: 10.3390/genes9080399

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sequence analysis results and transcription activation evaluation. (A) A classical motif (highlighted) was found in the amino acid sequence of ATH1. Both effector and reporter constructs were used in the transfection assays. (B) Transcriptional repression of ATH1 was identified and its repression function was increased by ATH1 interaction with OFP1 in vivo. The expression of 35S:luciferase (Luc) was used to normalized the expression of the GUS reporter gene. (C) The protein domain involved in ATH1 repression activation was identified by using a protoplast transfection system for checking relative GUS activity. LD, LexA DNA-binding domain; GD, Gal4 DNA-binding domain; HA, hemagglutinin.