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. 2018 Jul 25;10(8):243. doi: 10.3390/cancers10080243

Figure 2.

Figure 2

Total ERBB4 and ERBB4 variant expression. ERBB4 expression in 10 non-neoplastic normal brain (NNB), 28 glioblastoma (GBM), and 15 paired NNB and GBM samples. Expression levels were determined by RT-qPCR; total ERBB4 levels were standardized to H6PD mRNA, and variant levels were standardized to total ERBB4 mRNA levels. All measurements were made simultaneously but are displayed separately to aid comparison. Reference brain (Ref Brain) was a pooled brain sample from 7 donors (Ambion, Thermo-Fisher, Waltham, MA, USA) used for comparison. Total ERBB4 mRNA expression in Ref Brain, NNB and GBM samples (A). Total ERBB4 mRNA expression in paired NNB and GBM samples (B). Expression of JM-a (C) and JM-b (D) variants of ERBB4 in Ref Brain, NNB and GBM samples. Comparison of JM-a and JM-b expression levels in NNB (E) and GBM (F). (Note that the data shown in (E,F) are rescaled from (C) and (D).) CYT-1 and CYT-2 expression levels in NNB (G) and GBM (H). Experiments were performed in triplicate (* p < 0.05, Student’s t-test).