Table 1.
Currently commercially available high-throughput sequencing equipment.
| Platform | Maximum Read Length (bp) | Sequencing Output | Maximum Number of Reads per Run | Run Time | |
|---|---|---|---|---|---|
| PCR based HTS | |||||
| Illumina | iSeq 100 System | 2 × 150 | 1.2 Gb | 4 million | 9–17.5 h |
| MiniSeq System | 2 × 150 | 7.5 Gb | 25 million | 4–24 h | |
| MiSeq System | 2 × 300 | 15 Gb | 25 million | 4–55 h | |
| NextSeq 550 System | 2 × 150 | 120 Gb | 400 million | 12–30 h | |
| HiSeq 2500 System | 2 × 125/2 × 250 | 1000Gb/300 Gb | 4 billion/600 million | 29 h–6 days/7–60 h | |
| HiSeq 3000 System | 2 × 150 | 750 Gb | 2.5 billion | <1–3.5 days | |
| HiSeq 4000 System | 2 × 150 | 1500 Gb | 5 billion | <1–3.5 days | |
| HiSeq X Series | 2 × 150 | 1800 Gb | 6 billion | <3 days | |
| NovaSeq 6000 System | 2 × 150 | 6000 Gb | 20 billion | 16–44 h | |
| Ion Torrent | PGM (314 chip) | 200/400 | 50/100 Mb | 550 thousand | 2.3/3.7 hr |
| PGM (316 chip) | 200/400 | 300 Mb/1 Gb | 3 million | 3.0/4.9 hr | |
| PGM (318 chip) | 200/400 | 1/2 Gb | 5.5 million | 4.4/7.3 h | |
| Proton PI chip | 200 | 10 Gb | 80 million | 2–4 h | |
| Ion Gene Studio S5 (510 chip) | 200/400 | 0.5/1 Gb | 3 million | 4.5/10.5 | |
| Ion Gene Studio S5 (520 chip) | 200/400/600 | 1/2/1.5 Gb | 6/6/4 million | 7.5/12/12 h | |
| Ion Gene Studio S5 (530 chip) | 200/400/600 | 4/8/4.5 Gb | 20/20/12 million | 10.5/21.5/21 h | |
| Ion Gene Studio S5 (540 chip) | 200 | 15 Gb | 60 million | 19 h | |
| Ion Gene Studio S5 Plus (550 chip) | 200 | 25 Gb | 100 million | 11.5 h | |
| SMS NGS | |||||
| Pacific Biosciences | Sequel System | >1000 | 1–10 Gb | 500.000 | 0.5–10 h |
| PacBioRS II | >1000 (average 10.000) up to 60.000 | 1–10 Gb | 1–10 million | 0.5–6 h | |
| Oxford Nanopore | MinION | 5000 to >100.000 | 10–20 Gb | - | 48 h |
| GridIONx5 | 5000 to >100.000 | 50–100 Gb | - | 48 h | |
| PromethION | 5000 to >100.000 | 1.2 Tb | - | 48 h |
PGM: Personal Genome Machine.