(A) Schematic diagram of the genome organization of Botrytis cinerea mymonavirus 1 (BcMyV1). The coding strand of BcMyV1 is 7863 nt long and contains three Open reading frames (ORFs), and the ORF1 encode a protein L of 1968 amino acids (aa), possessing a mononegaviral RNA-dependent RNA polymerase (RdRp) domain and a mRNA-capping region V (Cap) domain. ORF2 and ORF3 encode two proteins of 169 aa and 250 aa, respectively. The black bars indicate the coding regions, and the gray bars represent the untranslated regions (UTRs) on the genome of BcMyV1. Two red arrowheads point out the positions of a 21 nt repeat region on the two UTRs, and the detailed sequence information are listed in (B). The numbers in the parentheses indicate the nt positions nearby the parentheses. (C) Multiple alignment of the amino acid sequences of RdRp in the protein L encoded by BcMyV1 and other (−ss)RNA viruses. “*” indicates identical amino acid residues; and “.” indicate low chemically similar amino acid residues. The abbreviations of virus names are listed in Table 1.