Figure 2.

Efficacy of LysK against dynamic S. aureus SA113 biofilms grown on glass surfaces in the Biostream flow cell. Biofilms were grown for 20 h at 19 °C under continuous flow of medium. Glass slides were then submerged in solutions of LysK at different concentrations or buffer as a control for 5 h (A,C) or 2 h (B,D), and stained with crystal violet. The stain was dissolved in 96% ethanol and the A_595nm measured spectrophotometrically (A,B). All values were normalized to the control. Error bars indicate standard deviations from at least three independent experiments. Bars with different letters are significantly different from each other (p < 0.05). Representative glass slides after washing and before solubilization of the stain are shown for the 5 h (C) and the 2 h (D) treatments.