Figure 1.

Subcellular distribution of SNAP-29 in synaptosomal fractions. (A) Crude synaptosomes (SS) were separated into fractions enriched in presynaptic cytosol, synaptic vesicles, and synaptosomal plasma membrane by sucrose gradient centrifugation. Ten micrograms of synaptosomal fractions on SDS/PAGE was sequentially immunoblotted with antibodies, as indicated, after stripping between antibody applications. (B) Synaptic vesicles purified by anti-synaptotagmin-conjugated affinity beads (anti-tagmin 1) were analyzed by SDS/PAGE and sequential immunoblotting with antibodies as indicated. Normal IgG-conjugated beads (IgG) were applied as a control.