Table 1.
Peptides identified by MALDI-TOF-MS in the RP-HPLC active fractions of camel milk casein hydrolysates. MALDI-TOF-MS = matrix-assisted laser desorption ionization time of flight mass spectrometry
| Fraction | Mass, m/z |
Sequence | Protein identity (fragment) | |
|---|---|---|---|---|
| Signal1 | Predict | |||
| P-CCP P1 | 913.12 | 913.44 | FIPYPNY | κ-casein (55 to 61) |
| 1,091.59 | 1,092.63 | RPKYPLRY | αs1-casein (1 to 8) | |
| 1,168.5 | 1,168.62 | TLTDLENLHL | β-casein (127 to 136) | |
| 1,861.12 | 1,862.93 | QIPQCQALPNIDPPTVE | κ-casein (72 to 88) | |
| 2,001.96 | 2,000.89 | MDQGSSSEESINVSQQKF | αs2-casein (4 to 21) | |
| P-CCP P2 | 1,059.76 | 1,059.51 | FFQLGDYVA | αs1-casein (159 to 167) |
| 1,092.70 | 1,092.63 | RPKYPLRY | αs1-casein (1 to 8) | |
| 1,140.48 | 1,139.57 | QDKIYTFPQ | β-casein (47 to 55) | |
| 1,321.06 | 1,322.66 | LHQGQIVMNPW | αs2-casein (78 to 88) | |
| 1,416.94 | 1,416.71 | MVPYPQRAMPVQ | β-casein (178 to 189) | |
| 2,006.91 | 2,009.06 | PFQEPVPDPVRGLHPVPQ | β-casein (193 to 210) | |
| P-CCP P3 | 2,169.84 | 2,170.19 | VRNIKEVESAEVPTENKISQ | αs2-casein (45 to 64) |
| 2,304.72 | 2,305.2 | SISSSEESITHINKQKIEKF | β-casein (15 to 34) | |
| 2,951.68 | 2,947.68 | QPKVMDVPKTKETIIPKRKEMPLLQ | β-casein (90 to 114) | |
P-CCP = pepsin digested camel casein proteins.
1
De novo peptide sequencing of the major RP-HPLC peaks of camel casein hydrolysates in MALDI-TOF-MS was performed by manual interpretation of the ion series.