Fig. 4.

Biolog Assay of Microbial Function. To screen microbial substrate utilization and tolerance, loop microbiota samples were inoculated in 96 well Biolog™ plates containing different metabolic substrates (sugars, amino acids) or environmental stressors (salts, pH, antibiotics). After 24 h, OD590 were recorded for plate well and Bray-Curtis dissimilarity was used to compare individual sample signatures. Two-way cluster dendrograms are displayed, which show microbiota in WT (w) and IL-10^−/− (IL) on AOX (a) and CONT (c) diet cluster similarly, regardless of host genotype, suggesting diet is the primary driver of microbial function.