Fig. 1.

PX-RICS is required for GABA_AR transport during chem-iLTP.

(A) WT and KO hippocampal neurons were treated with vehicle (mock) or moderately stimulated with NMDA in the presence or absence of KN93 as indicated, and surface (green) and intracellular (red) γ2 subunits were separately visualized. Scale bars, 10 μm.

(B–D) Quantitative analyses of the γ2 fluorescent signals in the distal dendrites (B). The number (C) and averaged size (D) of surface-expressed γ2 puncta within a 40-μm segment of the representative dendrites were also quantified.

Data are shown as the mean ± SEM. *P < .05; **P < .01; ***P < .001 (Student's t-test). n = 10 (WT) and 10 (KO) across two independent experiments. See also Supplementary Fig. 3