Fig. 4.
THP-1 cells stimulated by LAMPs extracted from M. pneumoniae. THP-1 cells were cultured in RPMI 1640 containing 10% FCS, 2 mM l-glutamine, 100 U/ml penicillin G, and 100 μg/ml streptomycin. The cell concentration of THP-1 was adjusted to 1 × 106 ml in 24-well plates. Different doses of LAMPs (0 μg/ml, 2 μg/ml, 4 μg/ml, 6 μg/ml, 8 μg/ml and 10 μg/ml) were co-cultured with THP-1 cells for 16 h. (a) Expression of HMGB1, TNF-α, IL-6 induced by LAMPs; (b) Expression of RAGE, TLR2, TLR4 induced by LAMPs. Data are shown as mean ± SEM of 3 independent experiments. * p < 0.05; ** p < 0.01