Fig. 3.

Cleavage of Ng1–78-Myc-DDK fusionprotein by calpain-1; a Coomassie stained SDS PAGE gel, Lane 1, size markers; lane 2, Ng-Myc-DDK substrate; lanes 3–6, digests with 3.3 to 90-fold diluted calpain stock solution; lane 7, calpain control; b Western blot of calpain-1 Ng1–78-Myc-DDK fusion protein digests by antibody NG22. Lane 1, Ng-Myc-DDK substrate; lanes 2–4, digests with 3.3, 30 and 90-fold diluted calpain stock solution; lane 5, calpain control. The positions of the substrate and of the main cleavage product are indicated