Fig. 2.

Comparative genomics of the plasmids carrying mcr-3 and/or mcr-1 genes.

A. Genomic map of the mcr-1-bearing plasmid pGDZJ003_mcr-1 that coexists with the mcr-3.7-harboring plasmid pGDZJ003_mcr-3.7 in E. coli.

B. Schematic representation for circular genome of the mcr-3.7-harboring plasmid pGDZJ003_mcr-3.7.

Circles (from inside to outside) separately denote the GC screw, GC content and the open-reading frames in both DNA strands [27]. The plasmid sequences were annotated by RAST, and the maps were generated using Circos program [27].

C. Colinear genomic analyses of three plasmids pGDZJ003_mcr-1, pSH146–32 and pCSZ4.

D. Linear genome alignment of the mcr-3-positive plasmid pGDZJ003_mcr-3.7 with the other two plasmids (pMCR3_WCHEC-LL123 and pMCR_1511).