Comparison of growth curves of E. coli with or without carrying mcr-like genes.
Growth curves of two negative control strains, the E. coli MG1655 alone (A) or carrying the empty vector pBAD24 (B).
C. Arabinose-induced expression of the neisserial EptA inhibits the growth of E. coli MG1655.
D. Expression of MCR-1 exerts effect on the growth of E. coli MG1655.
E. Expression of MCR-2 interferes the growth of E. coli MG1655.
F. The presence of MCR-3 inhibits the growth of E. coli MG1655.
The expression of eptA and/or mcr-1 (mcr-2 & mcr-3) was triggered through the addition of different levels of arabinose (0.00%, 0.02%, and 0.20%, w/v) into LB media. The means of three independent replicates were shown and the error bars represent the standard deviation (SD).