Fig. 3.

OGN over-expression recruited more CD8+ cells in xenograft models.

(a1) Representative image from MC38 OGN-over-expression (green) displayed a significant increase in CD8+ cells recruitment (red), while the control group (a2) recruited much fewer CD8+ cells. The Quantification was shown in c1. (b2) VEGF expression was reduced in the OGN over-expression group in xenograft compared to the control group (b1). The Quantification was shown in c2. The gross images and the H&E staining of Matrigel plug showing the number of blood vessel formation was markedly reduced in OGN over-expression group (d2) compared to the control group (d1). Immunofluorescent staining of CD31 (endothelial cell marker) in OGN group (e2) was much weaker than the control group (e1). The Quantification was determined by Student's t-test in f1-f2.