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. 2018 Aug 29;49:83. doi: 10.1186/s13567-018-0575-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Laying performance of challenged and control birds in experiment 1. Eggs were collected throughout the experiment, three times a day, keeping handling of animals to the minimum, in order to avoid the occurrence of stressful events. We compared the egg production between challenged and control birds on a weekly basis, adopting the Wilcoxon matched-pairs signed-ranks on daily mean values for each group. Significance level was set at P < 0.05. Data are shown here as mean ± standard deviations. The differences in the weekly egg production between the two groups were significant (P < 0.05) for 9 weeks after the challenge.