Table 2.
Virus detection and histopathological damage in the different sections of the oviducts collected from day 4 to day 10 pi
| Day post-infection | 4 | 6 | 10 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| RRT-PCR positive/total (%)a | IHC score (%)b | Histopathology score (%)c | RRT-PCR positive/total (%) | IHC score (%) | Histopathology score (%) | RRT-PCR positive/total (%) | IHC score (%) | Histopathology score (%) | |
| Infundibulum | 5/7 (71) | 0.85 ± 1.46 (29) | 0.51 ± 0.63 (43) | 4/7 (57) | 0.85 ± 1.21 (43) | 0.66 ± 0.77 (57) | 1/7 (14) | 0.14 ± 0.38 (14) | 0.14 ± 0.19 (43) |
| Magnum | 4/7 (57) | 0.43 ± 0.79 (29) | 0.17 ± 0.31 (29) | 3/7 (43) | 0.57 ± 1.13 (29) | 0.11 ± 0.30 (14) | 1/7 (14) | 0.00 (0) | 0.02 ± 0.07 (14) |
| Uterus | 4/7 (57) | 0.71 ± 1.25 (29) | 0.00 (0) | 3/7 (43) | 0.14 ± 0.38 (14) | 0.22 ± 0.35 (57) | 1/7 (14) | 0.00 (0) | 0.00 (0) |
In this table, we report the overall detection of virus RNA along with the presence of the virus nucleoprotein and the associated histological lesion, in the infundibulum, magnum and uterus of birds of the first experiment. RRT-PCR positive samples were tested by IHC and a score was obtained as follows: 0 no staining, 1 small clusters of positive cells, 2 multiple accumulations of positive cells and 3 extensive staining. Irrespective of RRT-PCR, all samples were examined for histological lesions taking into account the presence of: (a) heterophilic infiltrate; (b) lymphocytic and plasmacytic infiltrate; (c) hemorrhage and/or edema; (d) necrosis; (e) hemorrhage and/or cell debris within the lumen. For each of these pathological features, a score was given as follows: 0 absence of the feature, 1 mild, 2 moderate, 3 severe. Scores are expressed as mean values ± standard deviations.
N.D.: not done.
aPercentage of RRT-PCR positive animals.
bPercentage of IHC positive animals.
cPercentage of animals with histopathological changes.