FIGURE 3.
Representative images showing estrogen receptor alpha (ERa)- and nNOS-immunolabeling in the hypothalamus of P11 infantile and P23 juvenile mice. nNOS (white labeling) and ERa (red nuclear labeling) are highly colocalized in the organum vasculosum laminae terminalis (OV), median preoptic nucleus (MEPO) (a), anteroventral periventricular nucleus of the hypothalamus (AVPV, b), and the ventromedial hypothalamic nucleus (VMH) in the tuberal hypothalamus (c), in both infantile and juvenile mice, as well as in the arcuate nucleus of the hypothalamus (ARH) in juvenile mice (c). Scale bar =100 im in (a) and (d), 150 im in (b) and 200 im in (c)