Fig. 3.

(A) Single cell sorting and TCR repertoire of activated CD8+ T cells shows dominant TCRA/B clonotype (TCRA CDR3: CAYRSGAFGNEKLTF; TCRB CDR3: CASSLDPGGSTEAFF) HLA-B*58:01+ allopurinol SJS/TEN blister fluid. Single cell TCR sequencing of CD8+CD137+ T cells from blister fluid. Circus plots represents CDR3 amino acids. (B) TCRβ CDR3 sequencing of bulk sorted PBMC showing that the dominant TCR clonotype in CD8+CD137+ T cells that was prevalent in blister fluid is rare in the peripheral blood. Abbreviations: TCR, T-cell receptor; SJS/TEN, Stevens-Johnson syndrome/toxic epidermal necrolysis; PBMC, peripheral blood mononuclear cell.