Skip to main content
. 2018 Aug 23;9:470. doi: 10.3389/fendo.2018.00470

Figure 4.

Figure 4

Endogenous production of 27HC is not a major contributor in H1395 cell proliferation. (A) CYP27A1 gene expression (n = 4). (B) Cell proliferation assay using H1395 cells with shRNA against CYP27A1 (n = 4). Open bars, vehicle treatments; closed bars, 27HC (1 μM) treatments. (C) CYP7B1 gene expression (n = 4). (D). cell proliferation assay using H1395 cells with siRNA against CYP7B1 (n = 4). Open bars, vehicle treatments; closed bars, 27HC (1 μM) treatments. *p < 0.05 against vehicle control. (E) Effect by clotrimazole on the 27HC-induced cell proliferation. Cells were treated with vehicle or 27HC (1 μM) in the absence (Veh.) or presence of clotrimazole (1 μM) for 24 h (n = 4). *p < 0.05 vs. vehicle control.