Table 1.
Selected genes, minimal inhibitory concentrations (MIC), and % changes in resistance
| Overexpressed ORF | MIC (μg/mL) with ORF | Increase in MIC (%) |
|---|---|---|
| Cadmium | 100a | |
| cmd | 125 | 30 |
| hdfR | 125 | 30 |
| napA | 125 | 30 |
| napF | 125 | 30 |
| fliN | 125 | 30 |
| Cobalt | 350a | |
| fliZ | 500 | 40 |
| zitB | 500 | 40 |
| cysE | 450 | 30 |
| yqiA | 450 | 30 |
| acp | 400 | 10 |
| yfgG | 400 | 10 |
| yhhI | 400 | 10 |
| ynaE | 400 | 10 |
| Copper | 400a | |
| pnp | 700 | 80 |
| fliZ | 600 | 50 |
| nusA | 600 | 50 |
| acrB | 500 | 30 |
| iraD | 500 | 30 |
| yfdN | 500 | 30 |
| yncN | 500 | 30 |
| gabt | 450 | 10 |
| lysR | 450 | 10 |
| nudJ | 450 | 10 |
| nudL | 450 | 10 |
| yciF | 450 | 10 |
| Nickel | 550a | |
| cysE | 700 | 30 |
| acpH | 650 | 20 |
| acpT | 650 | 20 |
| ybjC | 650 | 20 |
| yedN | 650 | 20 |
| yfeH | 650 | 20 |
| y S | 650 | 20 |
| yhbS | 600 | 10 |
| Silver | 5a | |
| rbfA | 10 | 100 |
| ydcQ | 10 | 100 |
| lysM | 10 | 100 |
| yaaY | 10 | 100 |
| yaiI | 20 | 300 |
| ynaE | 20 | 300 |
| Zinc | 200a | |
| ZitB | 400 | 100 |
| hlyD | 300 | 50 |
| yhil | 300 | 50 |
| rfaJ | 250 | 30 |
| ydcQ | 250 | 30 |
| ynaE | 250 | 30 |
| cysE | 250 | 30 |
| iraD | 250 | 30 |
| PerC | 250 | 30 |
| yfdN | 250 | 30 |
The ORFs selected in the presence of toxic concentrations of transition metals, the concentration at which the gene was selected, and the % change in resistance. MIC measurements were reproduced in triplicate. (Genotypes are explained in Supplementary Material)
a
MIC (μg/mL) determined using the control plasmid expressing lacZ