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Knockout strains with increased sensitivity to metal toxicity
| Gene KO | Toxin | WT MIC (mM) | KO MIC (mM) |
|---|---|---|---|
| yqiA | CoCl2 | 1.0 | 0.8 |
| pnp | CuCl2 | 1.8 | 1.4 |
| hdfR | CdCl2 | 0.5 | 0.4 |
Each KEIO knockout strain has a kanamycin cassette inserted into the ORF whose function it knocks out. Listed here are the KO strains, the toxin, and the WT and KO MIC. (MIC measurements were reproduced in triplicate.)
