Figure 4.

The effect of horse oil, hydrolysed horse oil or sophorolipid on wound‐healing potential measured using an in vitro cell culture model (cell migration assay). Effects of different concentrations of horse oil (25–100 μg ml⁻¹), hydrolysed horse oil (0.5–5 μg ml⁻¹) or sophorolipids (0.5–5 μg ml⁻¹) on human skin fibroblast migration were examined using an Oris Cell migration assay kit. Fibroblasts were cultured at a density of 1 × 10⁴ cells/well in 96 well plates at 37°C for 24 h, the medium was substituted with serum‐free medium containing different concentrations of horse oil, hydrolysed horse oil (Hyd. HO) or sophorolipids, and culturing continued at 37°C for 6 days. After washing with 1× phosphate‐buffered saline (PBS), cells were observed by microscopy. Images are representatives of at least six samples of each group (duplicate samples in each experiment and three separate experiments respectively).