Table 1.

Selection of 29 recombinant proteins by serological initial screening and final confirmation

Proteins	Annotation	AUCa	95% CI	Ratiob
PTB versus HC
Rv3403c	Hypothetical protein	0.8537	0.7779–0.9294	3.04
Rv0222	Probable enoyl‐CoA hydratase EchA1	0.8129	0.7280–0.8979	2.01
Rv3875	6 kDa early secretory antigenic target EsxA	0.7429	0.6565–0.8492	2.20
Rv0309	Possible conserved export protein	0.7374	0.6407–0.8340	2.56
Rv2657c	Probable PhiRv2 prophage protein	0.7353	0.6346–0.8360	2.50
Rv3872	PE family related protein PE35	0.7277	0.6302–0.8253	2.03
Rv2649	Probable transposase for insertion sequence element	0.7206	0.6202–0.8209	2.81
Rv1509	Hypothetical protein	0.7111	0.6129–0.8093	3.40
Rv3428c	Possible transposase	0.6894	0.5866–0.7923	2.24
Rv1256c	Probable cytochrome P450 130 Cyp130	0.6655	0.5641–0.7667	2.36
Rv2660c	Hypothetical protein	0.6590	0.5524–0.7657	2.09
Rv1573	Probable PhiRv1phage protein	0.6227	0.5337–0.7117	2.18
Rv3874	10 kDa culture filtrate antigen EsxB	0.6011	0.4916–0.7106	4.50
Rv3402c	Conserved hypothetical protein	0.5714	0.4623–0.6804	3.12
Rv3405c	Possible transcriptional regulatory protein	0.5690	0.4811–0.6569	2.40
Rv1972	Conserved Mce associated membrane protein	0.5562	0.4481–0.6642	2.53
Rv1981c	Ribonucleside‐diphpsphate reductase	0.5517	0.4422–0.6612	2.51
Rv3427c	Possible transposase	0.5447	0.4371–0.6523	2.58
Rv1964	Conserved hypothetical integral membrane protein	0.5381	0.4276–0.6486	2.70
EPTB versus HC
Rv0222	Probable enoyl‐CoA hydratase EchA1	0.7523	0.5745–0.9302	2.04
Rv3403c	Hypothetical protein	0.7386	0.5974–0.8791	3.35
Rv1514c	Conserved hypothetical protein	0.6653	0.5474–0.7832	3.32
Rv2652c	Probable PhiRv2 prophage protein	0.6150	0.4885–0.7416	2.03
Rv2658c	Possible PhiRv2 protein	0.6141	0.4903–0.7379	2.74
Rv1577c	Probable PhiRv1phage protein	0.5946	0.4687–0.7205	2.60
Rv1767	Conserved protein	0.5722	0.4433–0.7010	2.47
Rv1508c	Probable membrane protein	0.5468	0.4195–0.6741	3.34
Rv0309	Possible conserved export protein	0.5464	0.4137–0.6791	2.04
Rv1255c	Probable transcriptional regulatory protein	0.5243	0.3915–0.6570	2.10
Rv1770	Conserved protein	0.5187	0.3869–0.6505	2.03
Rv3400	Probable hydrolase	0.5098	0.3791–0.6405	2.72
Rv1985c	Probable transcriptional regulatory protein	0.5044	0.3767–0.6321	3.55

a. AUC values with the 95% CI were used to confirm the potential diagnostic markers obtained by the initial screening with ELISA.

b. The ratio of OD₆₃₀ between PTB (EPTB) and healthy control (HC) when the indirect ELISA was used to initially screen the potential diagnostic markers.