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. 2018 Aug 13;365(18):fny198. doi: 10.1093/femsle/fny198

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© FEMS 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Immunoblot of Strep(II)-tagged NpDps proteins. Total protein extracts of strains; control, OENpDps2 and OENpDps5 were analysed using chemiluminescent detection of the Strep(II)-tag, showing the presence of NpDps2-strep(II)tag and NpDps5-strep(II)tag fusion proteins from cells cultured at different light intensities. From left to right: MW ladder (PageRuler); in sets of three lanes—control strain, the OENpDps2 strain and the OENpDps5 strain. Three samples from each strain were analysed: before treatment (1), after 5 days under 60 μmol photons m⁻² s⁻¹ (2), and after 5 days under 500 μmol photons m⁻² s⁻¹ (3). The bands at 20 kDa (A) and 18 kDa (B) indicate the NpDps2-strep(II)tag fusion protein and NpDps5-strep(II)tag fusion protein, respectively.