Figure 2. The stimulatory effect of estradiol is mediated by an intracellular receptor and does not involve GPR30.
A,B Representative examples of GnRH neurons responding to 15min exposure to 100pM (A) or 100nM (B) E2. C Representative example of a GnRH neuron responding to 5 min exposure to 100nM E2. D,E Representative traces of GnRH neurons that did not respond to 100 nM E2-6-BSA (D) 100 nM 17α-estradiol (E) or 100nM G-1 (F). All of these three cells responded to a caffeine (caf) stimulus at the end of the experiment.