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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: ACS Synth Biol. 2018 Jan 12;7(2):443–451. doi: 10.1021/acssynbio.7b00225

Figure 5.

Figure 5

Smad2 phosphorylation responses in optoTGFBRs-HeLa cells. (A) Time course of P-Smad2 response to 100 pM TGF-β1 ligand stimulation. (B) Time course of P-Smad2 response to continuous blue light illumination (488 nm, 0.8 mW/cm2). (C) P-Smad2 response at 45 min after different power of blue light stimulations. Relative P-Smad2 level was quantified as the ratio of P-Smad2 to corresponding Smad2 signal (normalized with the maximal signal in each time course). Error bars show the standard deviations from three replicates.