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. 2018 Aug 30;13(8):e0201595. doi: 10.1371/journal.pone.0201595

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© 2018 Yang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Nasal fibroblasts were pretreated with the following drugs and specific inhibitors: apigenin (5 μM), SB203580 (10 μM), SP600125 (5 μM), and BAY11-7082 (3 μM) and then stimulated with TGF-β1 (5 ng/ml). (A) Migration of fibroblasts was assessed by a wound scratch assay. The images were obtained by a bright field microscope. The wound area was measured using an ImageJ analyzer. (B) Invasion of fibroblasts was assessed by a transwell invasion assay. The number of fibroblasts was measured using an ImageJ analyzer. (C) Contractile activity was assessed by collagen gel contraction assay. The contraction area was measured using an ImageJ analyzer. Results were obtained from at least three independent experiments. * p <0.05 vs. TGF-β1; ^†p < 0.05 vs. apigenin.