Figure 3.

Septation of the vegetative mycelium in wild-type (WT) and mutant strains as indicated. (A) Cartoon to indicate the three types of septa described in this investigation: septa in trunk hyphae (1), in the base of branches (2), and in ascogonia (3). (B) Septation in vegetative mycelium of the wild-type and ΔSmKin3^sos1− strains after 16, 24, and 48 hr of incubation on BMM (solid malt–cornmeal fructification medium). (C) Complementation strains show WT-like hyphal septation. ΔSmkin3::NAkin3 and ΔSmkin3::OEkin3 indicate transformants, where fertility was restored by ectopic integration of the WT Smkin3 gene under the control of a native (NA) or overexpression (OE) promoter. The arrows indicate multiple narrow-spaced septa in the strain overexpressing Smkin3. Mycelium samples were grown on BMM medium for 2 days and stained with Calcofluor White M2R. (D) Quantification of the hypersepation phenotype in strains as indicated. Error bars show SD of two experiments (n = 100).