Figure 4.

Sexual development of wild-type (WT) and mutant strains. Strain denomination is the same as in Figure 2. Ascogonia, unpigmented and pigmented protoperithecia, as well as perithecia develop after 2, 3, 4, and 7 days, respectively, on complete solid malt–cornmeal fructification medium. Ascogonial coils were stained with Calcofluor White M2R and white arrows indicate septa. Bars: black represents 20 μm and white represents 100 μm.