Skip to main content
. 2018 Jul 16;210(1):137–153. doi: 10.1534/genetics.118.301261

Table 2. Plasmids used in this study.

Plasmid Feature Reference
pRS426 URA3, lacZ_a, bla Christianson et al. (1992)
pDrivehph trpC(p)::hph, lacZ_a, bla, kann Nowrousian and Cebula (2005)
pKO4490 Deletion cassette for Smkin3, 1 kb 5′ flank, hph from pDrivehph and 1 kb 3′ flank in pRS426 This study
pRSnat trpC(p)::nat, URA3, bla Klix et al. (2010)
pDS23 gpd(p)::egfp::trpC(t), trpC(p)::nat, URA3, bla Schindler and Nowrousian (2014)
pMHN2 gpd(p)::mrfp::trpC(t), trpC(p)::hph, bla Rech (2007)
pNAkin3 Smkin3 with 1,9 kb upstream and 1,1 kb downstream sequence in pRSnat This study
pOEkin3 gpd(p)::mrfp::SmKin3::trpC(t) in pRSnat This study
p4490-K26R pNAkin3 carrying Smkin3K26R mutation This study
p4490-K39R pNAkin3 carrying Smkin3K39R mutation This study