| KO-4490-5fw |
gtaacgccagggttttcccagtcacgacggaa |
SmKin3 5′ flank with |
| ttccttccccatcgcgctaccaggtaca |
pRS426 overlap |
| KO-4490-5rv |
cgagggcaaaggaatagggttccgttgaggct |
SmKin3 5′ flank with |
| tttggttacagaagggtgattgt |
hph overlap |
| KO-4490-3fw |
gcccaaaaatgctccttcaatatcagttgctgag |
hph with SmKin3 3′ flank |
| gtgatgaatggtgaagagaag |
overlap |
| KO-4490-3rv |
gcggataacaatttcacacaggaaacagcgaatt |
SmKin3 3′ flank with |
| cctgttttggttactgtaacagccgt |
pRS426 overlap |
| SmKin3_F |
atggccgacgaaggagtcgc |
SmKin3 start |
| SmKin3_R |
ctaagatccggcaacagccc |
SmKin3 end |
| kin3- 5-2DR |
aaccgtgtacttcgattggc |
SmKin3 5′ flank |
| kin3-3-DR2 |
aagcttgatcgccatggca |
SmKin3 3′ flank |
| hph1MN |
cgatggctgtgtagaagtactcgc |
hph |
| hph2MN |
atccgcctggacgactaaaccaa |
hph |
| 5′na4490-fw |
gacggtatcgataagcttgatatcgggtcctcaacggcgcacttg |
pRS426, SmKin3 5′ UTR |
| 3′na4490-rv |
cggccgctctagaactagtgcaacgtaggta |
SmKin33′ UTR, pRS426 |
| tgtacgtagctgc |
| Pgpd-mrfp-fw |
catcgcagcttgactaacagctacatggcctcctccgaggacgtcatc |
gpd(p), mrfp
|
| mrfp-kin3-rv |
gtcggccatagaaccaccaccaccggcgccg |
mrpf, SmKin3
|
| gtggagtggcg |
| mrfp-kin3-fw |
cggcgccggtggtggtggttctatggccgacgaaggagtcgccaac |
mrpf, SmKin3
|
| kin3-Ttrpc-rv |
gtggatccactagttctagactaagatccggc |
SmKin3, trpc(t) |
| aacagccccccaccg |
| kin3K26R-Q5-fw |
cgtttacaggggaattgacagg |
SmKin3 K26 codon |
| kin3K26R-Q5-rv |
acaccaaaactgcctcctgtg |
SmKin3 K26 codon |
| kin3K39R-Q5-2fw |
gtggccatcagacatgtacg |
SmKin3 K39 codon |
| kin3K39R-Q5-2rv |
tgtttcgcccgttgtcctg |
SmKin3 K39 codon |
| pro11mut-fw |
cgtatgggaaagaggaaggg |
pro11 before pro11 mutation |
| pro11mut-rv |
cggggccttgtgtttgatc |
pro11 after pro11 mutation |
| pro11-21 |
aagcgcgcttgccagtcgctgc |
pro11 5′ flank |
| pro11-kor |
acgatcagcctcggaaagaccgc |
pro11 3′ flank |
| pro22-ClaI-for |
gcggggtttttcagtatctacgattcatctgcagctaaccaaacattgag |
pro22 gene |
| atcaaccccag |
| pro22-SacI-rv |
cctccctagcgatgaggatgatgacatggtaaccattattggacctcaag |
pro22 gene |
| accacgtg |
| pro22vp1 |
ccaagttcagcaacaagaggatgg |
pro22 5′ flank |
| pro22vp2 |
cttacggtagctacaacccgtaca |
pro22 3′ flank |
