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. 2018 Jul 11;210(1):189–201. doi: 10.1534/genetics.118.301272

Figure 3.

Figure 3

Tissue specificity of the fat-6 and fat-7 requirements. (A) Only paqr-2(+) expressed from its native promoter can significantly restore the expression levels of the fat-7::gfp reporter in the paqr-2 mutant (1-day-old adult worms; n ≥ 20). (B–E) Expression pattern of the CRISPR-modified fat-6 locus fused to GFP at the 3′ end of the coding region. Note the strong intestinal expression (arrowheads in C) as well as the distinct hypodermal cell expression in a different focal plane shown in the enlarged insets in D and E (arrowheads). (F) Tail tip phenotype of 1-day-old transgenic worms with the indicated genotypes. Both fat-6 and fat-7 contribute to the ability of the Ppaqr-2(+) transgene to suppress the tail tip phenotype of the paqr-2 mutant (n ≥ 100). (G) Length of 1-day-old adult worms with the indicated genotypes cultivated on normal plates (NGM) or 20 mM glucose (n ≥ 20). (H) Brood size of worms with the indicated genotypes cultivated on normal plates (n = 10). * P < 0.05, ** P < 0.01, and *** P < 0.001.