Figure 2.

SGE1 alleles from BY and 378 determine [C₂C₁im]⁺-tolerance phenotypes. Haploid BY or diploid 378 strains harboring (A and B) sge1Δ or (C and D) ilt1Δ null mutations were transformed with plasmids containing the indicated SGE1 or ILT1 sequences. In (A and B), a plasmid containing site-directed mutations in the BY SGE1 gene sequence (BY SGE1^PLL) was included. Transformed strains were then cultured aerobically in tubes containing 10 ml YPD (pH 5), 125 mM [C₂C₁im]Cl, and the appropriate antibiotic. Average cell densities (OD₆₀₀) ± SD are reported from three independent biological replicates.