Figure 4.

The SGE1^PLL allele confers greater tolerance to [C₂C₁im]Cl than the SGE1^SLS variant. (A) The individual S. cerevisiae strains were grouped based on their SGE1 genotype and plotted according to their relative growth in 250 mM [C₂C₁im]Cl. For strains containing SGE1^PLL and additional SGE1 SNPs, the amino acid changes in each strain are relative to S288c reference sequence. The numbers of strains for each genotype are listed in parentheses. Statistical significance of growth differences between strains containing the SGE1^PLL alone and SGE1^SLS alleles was determined by unpaired Student’s t-test. * P < 2e⁻¹⁰. (B) Sge1 protein sequences encompassing the region surrounding the Sge1^SLS/Sge1^PLL variants from different strains and species from the genus Saccharomyces were aligned to display the amino acid sequences at the corresponding SLS/PLL residues. For each species, their locations of isolation as well as common strain identifiers are listed. (C) Average ± SEM aerobic cell densities of BY SGE1^SLS cells or the indicated SGE1 mutations introduced into the genome by CRISPR/Cas9 from three biological replicates are shown. (D) Average total cell growth + SD was normalized relative to growth for BY SGE1^SLS strain after 10 hr of culturing and plotted with SD from three independent biological replicates. Statistical significance determined by paired Student’s t-tests. * P < 0.05.