Figure 6.

Plasma membrane-localized Sge1^PLL protein functions independently of ILT1. BY strains containing GFP fused to the indicated genes were cultured in YPD medium. (A) GFP fluorescence from representative cells was visualized with 100× magnification. Insets in the bottom right corners display a single representative cell with an additional 50% higher magnification. (B) ILT1 was deleted from BY strains containing SGE1^SLS or sge1^PLL alleles. Resulting strains were cultured in YPD (pH 5) medium containing 62.5 mM [C₂C₁im]Cl. Cell growth is reported as average cell densities ± SEM from three independent biological replicates.