Figure 5.

Preferential initiation of nuclear envelope reassembly at the poleward face of segregating chromosomes requires HP1a. (A) Stills from a time-lapse movie of a mitotic neuroblast expressing H2Av-RFP (red fluorescent protein, red) and Lamin-GFP (green) (see Movie S7). Nuclear envelope reassembly initiation is indicated by the green arrows. (B) Stills from a time-lapse movie of a mitotic neuroblast expressing H2Av-RFP (red), Lamin-GFP (green), and RNA interference (RNAi) against HP1a (see Movie S8). (C) Graph of location frequency for nuclear envelope reassembly initiation in wild-type (left) and HP1a RNAi (right) daughter neuroblasts. Asterisk indicates statistical significance by a χ² test (P = 0.04). (D) Graph of the time interval between anaphase onset and initiation of nuclear envelope reassembly in wild-type and HP1a-depleted neuroblast divisions. Each circle represents one division. (E) Graph of the percentage of divisions resulting in nuclear envelope deformities in wild-type and HP1a-depleted divisions. Time is written as min:sec after anaphase onset. Bar, 2 μm. See also Figure S3.