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Representative DMR traces for NOP receptor agonists (panels A-K) tested at 1 μM or at 0.1 μM (PWT2-N/OFQ, panel E), in the absence and presence of 200 ng/mL PTX. The effects of the same compounds are reported as DMR peaks in the absence and in the presence of 200 ng/mL PTX in panel L. Data in panel A-K are mean + sem of a single experiment performed in triplicate. Data in panel L are mean + SD of 3 separate experiments performed in triplicate.
