Table 2. Susceptibility profiles to different beta-lactams, detected extended-spectrum beta-lactamase (ESBL) genes, plasmid replicons and multi-locus sequence types (MLST) of ESBL-producing Enterobacteriaceae isolates from bean sprouts in the Netherlands.
| Species ID |
AMC | TZP | CTX | CAZ | ESBL genes | Plasmid replicons | MLST |
|---|---|---|---|---|---|---|---|
| K. pneumoniae | |||||||
| 13 | 8 | ≤4 | 4 | ≤1 | blaCTX-M-14 | IncFII,IncFIB(K),ColRNAI | ST-1296 |
| 5 | 16 | 8 | 8 | 2 | blaCTX-M-3 | IncFII(K) | ST-1565 |
| 19 | 4 | ≤4 | ≤1 | ≤1 | blaSHV-2 | IncFIA(HI1),IncFIB(K), IncHI1B,ColRNAI | ST-2176 |
| 16 | 8 | ≤4 | 8 | ≤1 | blaCTX-M-14 | IncFII,IncFIB(K),ColRNAI | ST-2657 |
| 6 | 16 | 32 | ≥64 | 4 | blaSHV-2 | IncFIA(HI1),IncR | ST-2658 |
| 20 | 16 | 16 | 8 | 2 | blaCTX-M-3, blaSHV-99a |
IncFIA(HI1),IncFIB(pKPHS1),IncFIB(K),IncFII(K),IncR,IncQ1,ColRNAI | ST-2659 |
| 21 | 16 | 8 | 8 | 2 | blaCTX-M-3, blaSHV-99a |
IncFIA(HI1),IncFIB(pKPHS1),IncFIB(K),IncFII(K),IncR,IncQ1,ColRNAI | ST-2659 |
| 18 | 16 | 8 | 32 | ≤1 | blaSHV-2 | IncFIA(HI1),IncR | ST-280 |
| 14 | 8 | 8 | 16 | 16 | blaCTX-M-27 | IncFIA(HI1),IncFIB(K) | ST-37 |
| 15 | 8 | 8 | ≥64 | 16 | blaCTX-M-27 | IncFIA(HI1),IncFIB(K) | ST-37 |
| 17 | 16 | 8 | 8 | 2 | blaSHV-2 | IncFIA(HI1),IncFII, IncFIB(K) | ST-39 |
| 8 | 8 | ≤4 | ≤1 | ≤1 | blaSHV-2 | IncFiA(HI1),IncR,ColRNAI | ST-392 |
| 7,9 | 4 | ≤4 | ≤1 | ≤1 | blaSHV-2 | IncFiA(HI1),IncR,ColRNAI | ST-392 |
| 11 | 16 | ≤4 | 8 | ≤1 | blaSHV-2 | IncFIA(HI1),IncR,ColRNAI | ST-45 |
| 12 | 8 | ≤4 | ≥64 | 8 | blaCTX-M-15 | IncFIB(K) | ST-45 |
| 10 | 8 | 8 | 2 | 2 | blaSHV-2 | IncFIA(HI1),Col(BS512),IncR, Col(MG828),ColRNAI | ST-485 |
| 4 | 16 | 8 | ≥64 | ≤1 | blaCTX-M-3, blaSHV-27 | IncFII(K),IncQ1 | ST-661 |
| 1,2,3 | 16 | ≤4 | 8 | ≤1 | blaCTX-M-3, blaSHV-27 | IncFII(K),IncQ1 | ST-661 |
| K. oxytoca | |||||||
| 29 | 8 | ≤4 | 8 | ≤1 | blaCTX-M-14 | IncFIA(HI1), IncFIB(pKPHS1),IncN | ST-195 |
| 33 | 8 | ≤4 | 4 | ≤1 | blaCTX-M-3 | IncN,IncU | ST-196 |
| 32 | 8 | ≤4 | ≥64 | 4 | blaCTX-M-27 | IncFIA(HI1),IncR | ST-2 |
| K. variicola | |||||||
| 34 | 8 | ≤4 | 8 | ≤1 | blaCTX-M-3 | IncN2,IncFIB(K),ColRNAI | ST-1142 |
| E. cloacae | |||||||
| 31 | ≥32 | 8 | ≥64 | 4 | blaCTX-M-15 | IncFII(pECLA),IncFIB(pENTE01), IncFIB(pECLA),ColRNAI | ST-144 |
aESBL genes called with a less than 100% identity and or length less than 100%. Minimum identity and length for call: 90.00% and 60% respectively. All plasmid replicons called by PlasmidFinder in default settings were reported. Shading within table indicates susceptibility interpretation according to EUCAST breakpoint table version 7.1, dark grey: resistant, light grey: intermediate and white: susceptible. ID isolate identification number, AMC amoxicillin-clavulanic acid, TZP piperacillin-tazobactam, CTX cefotaxim, CAZ ceftazidime, ESBL extended-spectrum beta-lactamase, MLST multilocus sequencing typing, ST sequence type.