Table 1. Induction of clnR is specific to LL-37-like cathelicidins.
Antimicrobiala | Relative clnR expressionb, c |
---|---|
LL-37 | 530.1 ±110.5 |
scrambled LL-37 | 1.7 ± 0.8 |
mCRAMP | 111.4 ± 27.3 |
SMAP-29 | 0.7 ± 0.1 |
Lysozyme | 1.1 ± 0.3 |
Ampicillin | 0.9 ± 0.2 |
Vancomycin | 0.9 ± 0.1 |
Nisin | 1.1 ± 0.4 |
Polymyxin B | 1.2 ± 0.2 |
aConcentrations of antimicrobials used: 2 μg/ml LL-37, 2 μg/ml scrambled LL-37, 2 μg/ml mCRAMP, 0.35 μg/ml SMAP-29, 1 mg/ml lysozyme, 4 μg/ml ampicillin, 0.5 μg/ml vancomycin, 7.5 μg/ml nisin, 200 μg/ml polymyxin B
bRelative expression determined by qRT-PCR and normalized to 630Δerm grown in BHIS without antimicrobials. Values are the mean of three replicates ± standard error of the mean.
cBolded values indicate significant difference (adjusted P value < 0.05) from 630Δerm grown in BHIS without antimicrobials and analyzed by one-way ANOVA and Dunnett’s test for multiple comparisons.