Figure 2.

Northern blots of MOR-1 variants. Northern blot was performed with total RNA from mouse brain by using a 121-bp exon 4 probe, a 141-bp exon 11 probe, and a 546-bp exon 2/3 probe generated by PCR with appropriate primers, as described in Materials and Methods. Briefly, ≈100 μg per lane of total RNAs were loaded and separated on the same 0.8% formaldehyde agarose gel, and transferred to a GenePlus membrane. The membranes hybridized with the exon-specific probes were exposed to the Kodak BioMax MS film for 24 h. The membranes stripped off the probes were rehybridized with a ³²P-labeled β-actin probe amplified by PCR with an amplimer set (CLONTECH) to estimate RNA loading.