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. 2018 Aug 20;14(8):e1007269. doi: 10.1371/journal.ppat.1007269

Fig 6. Effect of SIVcol Nef on SERINC5 expression and virion incorporation.

Fig 6

(A) The upper panel shows the infectivity of HIV-1 particles produced in HEK293T cells relative to the nef-defective control vector (100%). The lower panel shows Western blot analysis of lysates of the corresponding producer cells and purified virions. (B) Quantification of SERINC5 levels in virions determined by densitometry of Western blot analysis. Depicted are means (+SEM) from at least four independent experiments. (C) Surface and total cellular SERINC5 levels in Jurkat T cells containing an HA-coding sequence in exon 8 of the serinc5 alleles (corresponding to the predicted fourth extracellular loop of SERINC5) introduced by CRISPR/Cas9-assisted gene editing infected with NL4-3 IRES eGFP expressing the indicated Nef proteins. Cells were left untreated or treated with MG132 or ammonium chloride, which inhibit proteasomal and lysosomal degradation, respectively. Protein levels were determined at 2 days post-infection. Shown are means of four experiments (+SEM). *, p < 0.05; **, p < 0.01; ***, p < 0.001.