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. 2018 Aug 20;14(8):e1007269. doi: 10.1371/journal.ppat.1007269

Fig 9. Effect of SERINC5 antagonism on HIV-1 replication in PBMC cultures.

Fig 9

(A) Human PBMC were transduced with equal p24 amounts of VSV-G pseudotyped NL4-3 viruses carrying the indicated HIV/SIV nef gene or mutant thereof. Cells were activated with IL-2 and PHA either 3 days prior to transduction (preactivated) or 3 days and 6 days post infection (dpi). Every 3 days, samples of cell culture medium from triplicate wells were harvested to determine infectious virus yield and RT activity. (B) Relative infectivity of virions produced in PBMCs. Results were calculated by dividing infectious virus yield values obtained from TZM-bl reporter infectivity assay by corresponding RT activity values measured by RT radioactivity-based assay of the cell culture supernatants from day 12. (C) SERINC5 mRNA levels in PBMCs cultured in the presence of 2 μg/ml PHA and/or 10 ng/ml IL-2. Gene expression was measured through qRT-PCR and the values were normalized to internal GAPDH control as well as unstimulated PBMCs (CTRL) of the corresponding donor. Data shown represents measurements obtained from 4 donors (mean ±SEM). **, p < 0.01; ***, p < 0.001.