Figure 6.
Cellular bioenergetics analysis in A549 cells. (a) OCR profiles of cells without light-triggered CO release. (b) OCR profiles of cells after 1 h illumination to trigger CO release in situ. (c) Bioenergetics data of illuminated cells (1 h post CO release) including basal respiration, proton leak, ATP production, maximal respiration, reserve capacity, and non-mitochondrial respiration in the presence of 1 (red), 2 (blue) and TPP tail (green) at the 0.1–10 μM concentration range. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; AA+Rot, antimycin A and rotenone. Values represent the means ± SEMs from three independent biological experiments. The values that are significantly different compared to control by one-way ANOVA test are indicated by asterisks as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.