Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Genetic reagent (D. melanogaster)	R23E10-Gal4	Bloomington	RRID:BDSC_49032
Genetic reagent (D. melanogaster)	C5-Gal4	doi: 10.1002/ ssscne.22284		Paul Shaw Lab
Genetic reagent (D. melanogaster)	104y-Gal4	Paul Shaw Lab
Genetic reagent (D. melanogaster)	UAS-CsChrimson	doi: 10.1038/ nmeth.2836		provided by Vivek Jarayaman Lab
Genetic reagent (D. melanogaster)	UAS-2eGFP	Bloomington	RRID:BDSC_32186
Genetic reagent (D. melanogaster)	UAS-NaChBac	Bloomington	RRID:BDSC_9469
Genetic reagent (D. melanogaster)	UAS-syntaxin3-69	Fly Base	FBal0092503
Genetic reagent (D. melanogaster)	UAS-shibireTS	Paul Shaw Lab	Gene ID: 45928
Genetic reagent (D. melanogaster)	tubpGAL80ts	Bloomington	RRID:BDSC_7108
Genetic reagent (D. melanogaster)	UAS-INX6 RNAi	VDRC	v8638	Provided by the Chia-Lin Wu Lab
Antibody	Rabbit anti -INX6	Provided by the Chia-Lin Wu Lab		1:1,000
Antibody	Goat anti-mouse AlexaFluor488	Invitrogen	Catalog # A-10680	1:200
Antibody	Goat anti-rabbit AlexaFluor568	Invitrogen	Catalog # A-11011	1:200
Antibody	Goat anti-rabbit AlexaFluor647	Invitrogen	Catalog # A-21244	1:200
Antibody	Mouse anti-NC82	DSHB	AB_2314866	1:10
Antibody	Goat anti-rabbit AlexaFluor488	Invitrogen	Catalog # A-11008	1:200
Chemical compound, drug	Neurobiotin	Vector Labs	Cat. No: SP-1120
Software, algorithm	DART	bfklab		http://www.bfklab.com/
Software, algorithm	MATLAB code	This paper	142faca	https://github.com/melvynyap/gap-junction-sleep-control (copy archived at https://github.com/elifesciences-publications/gap-junction-sleep-control)
Chemical compound, drug	All-trans retinal	SIGMA-Aldrich	SID 24899355
Chemical compound, drug	Vectashield	Vector Labs	Cat. No: H-1000
Chemical compound, drug	Streptavidin	Invitrogen	Catalog number: S32357	1:200