Figure 2.

Impaired bioenergetics status in human ciPTEC-PC1KD. (A) The rate of mitochondrial ATP production was measured in human ciPTEC stably silenced for polycystin-1 (ciPTEC-PC1KD) vs. wild type clone (ciPTECwt), in the presence of the respiratory substrates GLU plus MAL (GLU/MAL, 5 mM each) or 5 mM SUCC plus 3 μM rotenone, or 5 mM ASC plus 0.5 mM TMPD (ASC/TMPD), as described in the legend of Figure 1. Values are means ± SEM (*P < 0.01 vs. ciPTECwt) obtained from three independent experiments and expressed as nmol NADPH/min per mg protein. (B) Cellular ATP levels were measured in ciPTEC-PC1KD vs. matched ciPTECwt, as described under Methods. Each histogram is representative of four independent experiments. Data are expressed as means ± SEM. Significant differences with respect to wt were calculated by Student's t-test (****P < 0.0001).